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rabbit pabs against human hdac-1/2/3/5/7 and vwf antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit pabs against human hdac-1/2/3/5/7 and vwf antibody
    Rabbit Pabs Against Human Hdac 1/2/3/5/7 And Vwf Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hdacs+1+5/anti+hdac1/pmc05338411-216-6-10
    Average 90 stars, based on 1 article reviews
    rabbit pabs against human hdac-1/2/3/5/7 and vwf antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts
    Article Snippet: Lysates were prepared in Laemmli buffer (Bio-Rad), and 25 μg protein/sample were separated using SDS-PAGE and transferred to either nitrocellulose (for O -GlcNAc immunoblots only) or PVDF membranes at a constant voltage of 100 V for 75 min for PVDF or constant amperage of 0.2A for 85 min. .. Equal protein loading was initially confirmed by Ponceau-S staining, and immunoblots were then probed for O -GlcNAc (CTD110.6, 1:5,000; Epitope Recognition and Immunodetection Facility, University of Alabama at Birmingham, Birmingham, AL; or RL-2, 1:2,000; ab2739; Abcam, Cambridge, MA), OGT (1:1,500; O6139; Sigma-Aldrich), Anti-NCOAT/OGA (1:1,000; sc66612; Santa Cruz Biotechnology, Santa Cruz, CA), skeletal α-actin (1:2,000; ab28052; Abcam), ANP (1:500; ab91250; Abcam), HDACs 1–5 (1:1,000; HDAC1: 5356; HDAC2: 5113; HDAC3: 3949; HDAC4: 5392; HDAC5: 2082; Cell Signaling, Danvers, MA), mSin3A (1:2,000; ab3479; Abcam), or REST (1:1,000; 07–579; Millipore, Billerica, MA). .. Blots were then stripped using 1 M NaOH and reprobed with GAPDH (1:1,500; ab9484; Abcam) and TATA binding protein antibodies (1:1,000; ab51841; Abcam) as purity and loading controls for the cytoplasmic and nuclear fractions, respectively, or calsequestrin (1:4,000; ab3516; Abcam) as the protein loading control for whole cell lysates.

    Western Blot:

    Article Title: Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts
    Article Snippet: Lysates were prepared in Laemmli buffer (Bio-Rad), and 25 μg protein/sample were separated using SDS-PAGE and transferred to either nitrocellulose (for O -GlcNAc immunoblots only) or PVDF membranes at a constant voltage of 100 V for 75 min for PVDF or constant amperage of 0.2A for 85 min. .. Equal protein loading was initially confirmed by Ponceau-S staining, and immunoblots were then probed for O -GlcNAc (CTD110.6, 1:5,000; Epitope Recognition and Immunodetection Facility, University of Alabama at Birmingham, Birmingham, AL; or RL-2, 1:2,000; ab2739; Abcam, Cambridge, MA), OGT (1:1,500; O6139; Sigma-Aldrich), Anti-NCOAT/OGA (1:1,000; sc66612; Santa Cruz Biotechnology, Santa Cruz, CA), skeletal α-actin (1:2,000; ab28052; Abcam), ANP (1:500; ab91250; Abcam), HDACs 1–5 (1:1,000; HDAC1: 5356; HDAC2: 5113; HDAC3: 3949; HDAC4: 5392; HDAC5: 2082; Cell Signaling, Danvers, MA), mSin3A (1:2,000; ab3479; Abcam), or REST (1:1,000; 07–579; Millipore, Billerica, MA). .. Blots were then stripped using 1 M NaOH and reprobed with GAPDH (1:1,500; ab9484; Abcam) and TATA binding protein antibodies (1:1,000; ab51841; Abcam) as purity and loading controls for the cytoplasmic and nuclear fractions, respectively, or calsequestrin (1:4,000; ab3516; Abcam) as the protein loading control for whole cell lysates.

    Immunodetection:

    Article Title: Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts
    Article Snippet: Lysates were prepared in Laemmli buffer (Bio-Rad), and 25 μg protein/sample were separated using SDS-PAGE and transferred to either nitrocellulose (for O -GlcNAc immunoblots only) or PVDF membranes at a constant voltage of 100 V for 75 min for PVDF or constant amperage of 0.2A for 85 min. .. Equal protein loading was initially confirmed by Ponceau-S staining, and immunoblots were then probed for O -GlcNAc (CTD110.6, 1:5,000; Epitope Recognition and Immunodetection Facility, University of Alabama at Birmingham, Birmingham, AL; or RL-2, 1:2,000; ab2739; Abcam, Cambridge, MA), OGT (1:1,500; O6139; Sigma-Aldrich), Anti-NCOAT/OGA (1:1,000; sc66612; Santa Cruz Biotechnology, Santa Cruz, CA), skeletal α-actin (1:2,000; ab28052; Abcam), ANP (1:500; ab91250; Abcam), HDACs 1–5 (1:1,000; HDAC1: 5356; HDAC2: 5113; HDAC3: 3949; HDAC4: 5392; HDAC5: 2082; Cell Signaling, Danvers, MA), mSin3A (1:2,000; ab3479; Abcam), or REST (1:1,000; 07–579; Millipore, Billerica, MA). .. Blots were then stripped using 1 M NaOH and reprobed with GAPDH (1:1,500; ab9484; Abcam) and TATA binding protein antibodies (1:1,000; ab51841; Abcam) as purity and loading controls for the cytoplasmic and nuclear fractions, respectively, or calsequestrin (1:4,000; ab3516; Abcam) as the protein loading control for whole cell lysates.

    Aqueous Normal-phase Chromatography:

    Article Title: Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts
    Article Snippet: Lysates were prepared in Laemmli buffer (Bio-Rad), and 25 μg protein/sample were separated using SDS-PAGE and transferred to either nitrocellulose (for O -GlcNAc immunoblots only) or PVDF membranes at a constant voltage of 100 V for 75 min for PVDF or constant amperage of 0.2A for 85 min. .. Equal protein loading was initially confirmed by Ponceau-S staining, and immunoblots were then probed for O -GlcNAc (CTD110.6, 1:5,000; Epitope Recognition and Immunodetection Facility, University of Alabama at Birmingham, Birmingham, AL; or RL-2, 1:2,000; ab2739; Abcam, Cambridge, MA), OGT (1:1,500; O6139; Sigma-Aldrich), Anti-NCOAT/OGA (1:1,000; sc66612; Santa Cruz Biotechnology, Santa Cruz, CA), skeletal α-actin (1:2,000; ab28052; Abcam), ANP (1:500; ab91250; Abcam), HDACs 1–5 (1:1,000; HDAC1: 5356; HDAC2: 5113; HDAC3: 3949; HDAC4: 5392; HDAC5: 2082; Cell Signaling, Danvers, MA), mSin3A (1:2,000; ab3479; Abcam), or REST (1:1,000; 07–579; Millipore, Billerica, MA). .. Blots were then stripped using 1 M NaOH and reprobed with GAPDH (1:1,500; ab9484; Abcam) and TATA binding protein antibodies (1:1,000; ab51841; Abcam) as purity and loading controls for the cytoplasmic and nuclear fractions, respectively, or calsequestrin (1:4,000; ab3516; Abcam) as the protein loading control for whole cell lysates.



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